The antioxidant activity of ethanolic extract of Vitex negundo Linn was examined by using DPPH and reducing power assay. The total phenolic contents of the ethanolic leaf extract 39 mg GAE/g and whereas flavonoid contents of these extracts were found to be 0.013 mg RE/g. The ethanol extract shows maximum inhibition (%) of 93.01% as compared to ascorbic acid who shows the maximum inhibition of (%) 93.50% and absorbance of 2.850 and 1.460 in reducing power assay at 1 µg/ml and 0.5 µg/ml respectively. To utilize these significant sources of natural antioxidants, further characterization of the phenolic composition is needed.
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